Question

Post transcriptional modification includes

• A. RNA editing
• B. Phosphorylation, Acetylation
• C. 5' capping, 3' poly adenylating and intron removal by splicing
• D. Ubiquitination

Hint:

• Post-transcriptional modifications occur primarily in eukaryotes to convert pre-mRNA into mature mRNA.
• Major modifications:
  • 5' cap (7-methylguanosine)
  • 3' poly(A) tail
  • Splicing (removal of introns, joining of exons)
• RNA editing is another, less universal, type of modification.

Answer 1

The Correct Option is C

Post-transcriptional modification refers to the process in eukaryotic cells where RNA transcripts (pre-mRNA) are modified before they are translated into proteins. This includes several key modifications:

1. 5' Capping: A modified guanine nucleotide is added to the 5' end of the RNA transcript. This cap protects the RNA from degradation and assists in ribosome binding during translation.
2. 3' Polyadenylation: A tail of adenine bases (poly-A tail) is added to the 3' end of the RNA. This modification also protects the mRNA from degradation and influences the stability and lifespan of the transcript.
3. Intron Removal by Splicing: Non-coding sequences (introns) are removed from the RNA transcript, and the remaining coding sequences (exons) are joined together. This process is essential for the correct translation of the genetic message.

Together, these modifications ensure the RNA molecule is stable and ready for translation into a protein. Of the provided options, 5' capping, 3' poly adenylating and intron removal by splicing accurately describe the main events involved in post-transcriptional modification.