Question

Oxidative phosphorylation is

• A. formation of ATP by transfer of phosphate group from a substrate to ADP
• B. oxidation of phosphate group in ATP
• C. addition of phosphate group to ATP
• D. formation of ATP by energy released from electrons removed during substrate oxidation

Answer 1

The Correct Option is D

Oxidative Phosphorylation

Oxidative phosphorylation is the final step in cellular respiration and occurs in the mitochondria. It is the process by which ATP (adenosine triphosphate) is synthesized using the energy released during the oxidation of nutrients, such as glucose and fatty acids. This energy is used to generate ATP, the primary energy carrier in cells. Let’s break this process down:

Process of Oxidative Phosphorylation:

1. Electron Transport Chain (ETC): - In the mitochondrial inner membrane, electrons from NADH and FADH₂ (produced earlier in glycolysis and the citric acid cycle) are transferred through a series of proteins known as the electron transport chain. - As electrons move through the chain, they release energy, which is used to pump protons (H⁺ ions) across the mitochondrial membrane into the intermembrane space. This creates a proton gradient. 2. Chemiosmosis: - The protons in the intermembrane space then flow back into the mitochondrial matrix through an enzyme called ATP synthase. - This flow of protons through ATP synthase provides the energy necessary for the phosphorylation of ADP to ATP. 3. Oxygen as the Final Electron Acceptor: - The electrons finally pass to oxygen, the final electron acceptor, where they combine with protons to form water (H₂O). - This ensures the electron transport chain keeps moving and continues the process of ATP production.

In this process, energy from the oxidation of molecules like glucose and fatty acids is used to generate ATP through the electron transport chain and ATP synthase. The overall equation of oxidative phosphorylation is:

\(NADH + H\)

Why is it called "Oxidative Phosphorylation"?

The term "oxidative" refers to the oxidation reactions that occur in the electron transport chain, where electrons are transferred from NADH and FADH₂ to oxygen. The term "phosphorylation" refers to the process of adding a phosphate group (via ATP synthase) to ADP to form ATP.

Key Features of Oxidative Phosphorylation:

• Occurs in the mitochondrial inner membrane.
• Involves the electron transport chain and ATP synthase.
• Energy is released from the oxidation of nutrients to drive ATP synthesis.
• Oxygen is required as the final electron acceptor.
• Produces a large amount of ATP (approximately 34 ATP molecules per glucose molecule).

Summary:

Oxidative phosphorylation is the process by which cells produce the majority of their ATP, using the energy released from the oxidation of nutrients to drive the synthesis of ATP via the electron transport chain and ATP synthase. This process requires oxygen and produces water as a byproduct.

Correct Answer: 

Option 4: Formation of ATP by energy released from electrons removed during substrate oxidation