Question

Match the tasks given in Group I with the associated techniques conventionally used as listed in Group II.

• A. P-2; Q-1; R-3; S-4; T-5
• B. P-3; Q-4; R-1; S-2; T-5
• C. P-5; Q-4; R-1; S-2; T-3
• D. P-3; Q-5; R-1; S-2; T-4

Hint:

When solving match-the-following in molecular biology, recall the {gold standard technique}: Bisulfite sequencing $⇒$ methylation, RNA-seq $⇒$ RNA discovery, FISH $⇒$ chromosomal/ploidy analysis, Exome sequencing $⇒$ SNPs, Density-gradient $⇒$ repetitive/satellite DNA.

Answer 1

The Correct Option is B

Step 1: Match each task to its conventional method
- P. Ploidy analysis: Done by fluorescence in situ hybridization (FISH) $⇒$ (3).
- Q. Profiling DNA methylation: Measured by bisulfite sequencing $⇒$ (4).
- R. Identifying non-coding RNAs: Uses RNA sequencing $⇒$ (1).
- S. Identifying SNPs: Typically done by exome sequencing $⇒$ (2).
- T. Satellite DNA isolation: Achieved by density-gradient centrifugation $⇒$ (5).
Step 2: Combine the matches
P-3; Q-4; R-1; S-2; T-5. Therefore, the correct answer is (B).