| (i) | Cell wall | (a) | Virulence factor |
| (ii) | Glycocalyx | (b) | Selective permeability |
| (c) | Attachment to surfaces | ||
| (d) | Protection from osmotic lysis |
The functions of the bacterial structures are matched based on their primary roles:
| Structure | Function |
|---|---|
| (i) Cell wall | (d) Protection from osmotic lysis |
| (ii) Glycocalyx | (a) Virulence factor, (c) Attachment to surfaces |
$\text{Explanation}$
Cell Wall (i) $\rightarrow$ (d) Protection from osmotic lysis: The bacterial cell wall (peptidoglycan layer) provides the structural rigidity necessary to counteract the high internal turgor pressure of the cell. This prevents the cell from bursting (osmotic lysis) when placed in a hypotonic solution.
Glycocalyx (ii) $\rightarrow$ (a) Virulence factor AND (c) Attachment to surfaces: The Glycocalyx is an outer layer of polysaccharides (and sometimes polypeptides).
Attachment (c): When thick and structured (capsule), it aids in adherence to surfaces and other cells, forming biofilms.
Virulence (a): A major function of the capsule form is to protect the bacterium from phagocytosis by immune cells, thereby increasing the pathogen's ability to cause disease (i.e., acting as a virulence factor).
The correct matches are (i)-(d) and (ii)-(a) and (c).
Comparing with the options, since the Glycocalyx has two major functions listed:
Option 2: (i)-(d), (ii)-(a) $\rightarrow$ Correct (Focusing on the virulence/protection function of the capsule).
Option 4: (i)-(d), (ii)-(c) $\rightarrow$ Correct (Focusing on the attachment function).
Both Option 2 and Option 4 represent valid primary functions for the Glycocalyx paired with the unique function of the Cell Wall.
$$\text{The correct matches based on standard microbiology are } \mathbf{(i)-(d)} \text{ and } \mathbf{(ii)-(a) \text{ and } (c)}$$
List I | List II | ||
|---|---|---|---|
| A | Clostridium butylicum | I | Ethanol |
| B | Saccharomyces cerevisiae | II | Streptokinase |
| C | Trichoderma polysporum | III | Butyric acid |
| D | Streptococcus sp. | IV | Cyclosporin-A |

Identify the taxa that constitute a paraphyletic group in the given phylogenetic tree.
The vector, shown in the figure, has promoter and RBS sequences in the 300 bp region between the restriction sites for enzymes X and Y. There are no other sites for X and Y in the vector. The promoter is directed towards the Y site. The insert containing only an ORF provides 3 fragments after digestion with both enzymes X and Y. The ORF is cloned in the correct orientation in the vector using the single restriction enzyme Y. The size of the largest fragment of the recombinant plasmid expressing the ORF upon digestion with enzyme X is ........... bp. (answer in integer) 