In Gram's staining technique, which acts as a mordant
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Iodine is crucial in Gram's staining as it helps the crystal violet stain to adhere more strongly to the bacterial cells, making the distinction between Gram-positive and Gram-negative bacteria clearer.
In Gram's staining technique, iodine acts as a mordant by forming a complex with crystal violet, which helps the dye penetrate the bacterial cell walls and retain the color during washing.