Okazaki fragments are short DNA segments synthesized on the lagging strand during replication. DNA ligase plays a critical role by sealing nicks between these fragments to form a continuous DNA strand. Other enzymes like helicase and primase have distinct roles, such as unwinding DNA and synthesizing primers, respectively
Arrange the following in correct order in polymerase chain reaction cycle:
I Deannealing
II Denaturation
III Extension
IV Annealing