To determine the genotype of a black-seed plant, a test cross is performed. A test cross involves crossing the organism with a homozygous recessive individual (bb).
- If the offspring produce both black and white seeds, the parent is heterozygous (Bb).
- If all offspring have black seeds, the parent is homozygous dominant (BB).
Thus, to determine the genotype of the black-seed plant, you must cross it with a plant having the recessive genotype bb.
The following diagram shown restriction sites in E. coli cloning vector pBR322. Find the role of ‘X’ and ‘Y’gens :