Step 1: In a pedigree chart, a circle typically represents a female and a square represents a male.
Step 2: A horizontal line connecting them with a specific symbol, such as $\bigcirc \! \! \! \! \! \! = \! \! \! \! \! \! \square$, indicates a consanguineous mating, meaning mating between relatives.
Step 3: This symbol does not represent unrelated mating, affected individuals, or twins.
Thus, the correct interpretation is mating between relatives (consanguineous mating).