Question

HbsAg is based on which principle

• A. Immunochromatography assays
• B. Chemiluminescence
• C. ELISA
• D. Immunofluorescence

Answer 1

The Correct Option is A

HbsAg, or Hepatitis B surface antigen, is commonly detected using Immunochromatography assays. This method involves the following principles:

1. Sample Application: A small sample, such as blood or serum, is applied to a test strip.
2. Capillary Action: The sample moves along the strip by capillary action.
3. Antigen-Antibody Reaction: If HbsAg is present, it binds to specific antibodies on the strip.
4. Visible Signal: The antigen-antibody interaction produces a visible line or signal, indicating a positive result for HbsAg.