Step 1: Understanding Genetic Engineering.
Genetic engineering involves the manipulation of an organism's DNA to introduce desirable traits, cure diseases, or enhance productivity.
Step 2: Applications in Medical Treatment.
In medicine, genetic engineering is used in gene therapy, production of insulin, vaccines, monoclonal antibodies, and treatment of genetic disorders.
Step 3: Applications in Agriculture.
In agriculture, it is used to create genetically modified (GM) crops such as Bt cotton, Bt maize, and Golden Rice, which are pest-resistant or nutritionally enriched.
Step 4: Conclusion.
Since genetic engineering is applied in both medical treatment and agriculture, the correct option is (C) Both (A) and (B).
Answer the following questions:
(a) [(i)] Explain how some strains of Bacillus thuringiensis produce proteins that kill certain insects such as lepidopterans but do not kill the Bacillus.
[(ii)] How is the above mechanism exploited for the production of Bt cotton plant by biotechnologists?
(b) [(i)] Explain how the amplification of gene of interest is done using PCR.
[(ii)] State two applications of the desired amplified fragment of DNA.
The basic scheme of the essential steps involved in the process of recombinant DNA technology is summarised below in the form of a flow diagram. Study the given flow diagram and answer the questions that follow.
(a) What is the technical term used for Step 4 in the above process?
(b) Which of the given two combinations of restriction enzyme should be used in Step 1? Justify your answer.
(i) EcoR I to cut the plasmid and Hind III to cut the alien DNA.
(ii) EcoR I to cut both the plasmid and alien DNA.