The processing of hnRNA into mRNA in eukaryotes involves the following steps:
- Capping: A methyl guanosine cap is added to the 5’ end of hnRNA, ensuring stability and aiding in ribosome recognition.
- Tailing: A poly-A tail is added to the 3’ end of hnRNA to prevent degradation and enhance translation.
- Splicing: Introns (non-coding regions) are removed, and exons (coding regions) are joined together to form a functional mRNA. This processing occurs in the nucleus of eukaryotic cells.
(a) What is the difference between native protein and denatured protein?