Question

Explain the features required to facilitate cloning into a vector.

Hint:

Remember: Essential vector features include Ori (replication), marker genes (selection), and MCS (insertion sites). % Topic Biotechnology and Genetic Engineering

Answer 1

A cloning vector must possess specific features to efficiently introduce foreign DNA into host cells for replication and expression. Key features of a cloning vector: \begin{enumerate} \item Origin of replication (Ori): Ensures self-replication within the host cell. \item Selectable marker: Allows identification of recombinant cells (e.g., antibiotic resistance genes). \item Multiple cloning sites (MCS): Contains restriction enzyme recognition sites for insertion of foreign DNA. \item Promoter and terminator sequences: Regulate gene expression within the host. \end{enumerate}