Question:

ELISA is used to detect viruses where the key reagent is

Updated On: May 19, 2024
  • alkaline phosphatase
  • catalase
  • DNA probe
  • RNase.
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The Correct Option is A

Solution and Explanation

ELISA (enzyme linked immunosorbent assay) screening test is the initial test to diagnose AIDS. The test works by detecting anitibodies/substances or protein which are produced in the blood when virus is present. Alkaline phosphatase and peroxidases are commonly I used enzymes as key reagent to perform the ELISA test. These enzymes are used to provide antibody- antigen complex in a specialised ELISA plate or tray. In ELISA test for detecting a particular antigen, its antibody is buffered and a drop of serum (supernatant of centrifuged blood) poured over it. If the latter contains antigen, it will produce antigen-antibody complex. A second enzyme labelled antibody is added. It forms enzyme-antigen-antibody complex, if the antigen is present. Substrate is now added. It produces a stain if the antigen is present.
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