Question

Chronic leukemia in children: Immunophenotyping.

Hint:

Immunophenotyping helps in identifying the leukemia subtype and tailoring therapy accordingly, especially in chronic leukemia cases.

Answer 1

Step 1: Importance of Immunophenotyping.
Immunophenotyping is used to classify leukemia based on the presence of specific cell surface markers using flow cytometry. This is essential for distinguishing between different types of leukemia and determining the appropriate treatment.
(1) Chronic Myelogenous Leukemia (CML): Cells typically express CD13, CD33, and CD15 markers.
(2) Chronic Lymphocytic Leukemia (CLL): Cells are usually positive for CD5, CD19, and CD23 markers, with low or absent expression of CD20.