Question:
Briefly explain the different steps involved in the construction of recombinant DNA.
Briefly explain the different steps involved in the construction of recombinant DNA.
Show Hint
Steps in rDNA: Isolation → Cutting → Insertion → Transformation → Selection.
Updated On: Mar 7, 2025
Hide Solution
Verified By Collegedunia
Solution and Explanation
Recombinant DNA (rDNA) construction involves combining DNA from different sources to create new genetic combinations.
The key steps involved in recombinant DNA technology include:
- Isolation of DNA: Extracting the desired gene from the donor organism.
- Cutting of DNA: Using restriction enzymes to cut DNA at specific sites.
- Insertion into a vector: Ligating the gene into a vector such as a plasmid.
- Transformation: Introducing the recombinant DNA into a host cell.
- Selection: Identifying cells that have successfully taken up the recombinant DNA.
Was this answer helpful?
0
0
Top Questions on Genetics
- Write a note on Colour blindness.
- Write a note on Thalassaemia.
- Explain the law of incomplete dominance with an example.
- What is the unit of heredity?
- In Klinefelter syndrome, the number of sex chromosomes is:
View More Questions
Questions Asked in UP Board XII exam
- If \(A = \begin{bmatrix} 3 & 1 \\ -1 & 2 \end{bmatrix}\), then show that \(A^2 - 5A + 7I = O\). Using this, obtain \(A^{-1}\).
- UP Board XII - 2025
- Matrices
- If \( y = \sin^{-1} x \), then prove that \( (1 - x^2) \frac{d^2y}{dx^2} - x \frac{dy}{dx} = 0 \).
- UP Board XII - 2025
- Differential Equations
- If \(A = \begin{bmatrix} 1 & 3 & 3 \\ 1 & 4 & 3 \\ 1 & 3 & 4 \end{bmatrix}\), then find \(A^{-1}\).
- UP Board XII - 2025
- Matrices
- Solve: \( (1 + x^2)\frac{dy}{dx} + 2xy - 4x^2 = 0 \).
- UP Board XII - 2025
- Differential Equations
- Prove that \(\int_0^\pi \sqrt{\frac{1+\cos 2x}{2}} \, dx = 2\).
- UP Board XII - 2025
- Calculus
View More Questions