Question:
Briefly explain the different steps involved in the construction of recombinant DNA.
Briefly explain the different steps involved in the construction of recombinant DNA.
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Steps in rDNA: Isolation → Cutting → Insertion → Transformation → Selection.
Updated On: Mar 7, 2025
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Solution and Explanation
Recombinant DNA (rDNA) construction involves combining DNA from different sources to create new genetic combinations.
The key steps involved in recombinant DNA technology include:
- Isolation of DNA: Extracting the desired gene from the donor organism.
- Cutting of DNA: Using restriction enzymes to cut DNA at specific sites.
- Insertion into a vector: Ligating the gene into a vector such as a plasmid.
- Transformation: Introducing the recombinant DNA into a host cell.
- Selection: Identifying cells that have successfully taken up the recombinant DNA.
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