Question:
Biolistics method is suitable for gene transfer into_________
Biolistics method is suitable for gene transfer into_________
Updated On: Apr 19, 2024
- Bacteria
- Plant cell
- Viruses
- Animal cells
Hide Solution
Verified By Collegedunia
The Correct Option is B
Solution and Explanation
During the transfer of genes in rDNA technology, the gene which is modified has to be taken up by the host cells. Many methods are used to transfer the modified genes into the host cell. One such method is the biolistic or gene gun method, which is suitable for plant cells. In this method, tiny metal balls (like micro-particles of gold or tungsten) coated with the recombinant gene are propelled into the plant cell at high velocity.
Was this answer helpful?
0
0
Top Questions on recombinant technology
Frequency of recombination between gene pairs on same chromosome as a measure of the distance between genes to map their position on chromosome, was used for the first time by
- NEET (UG) - 2023
- Biology
- recombinant technology
- Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?
- NEET (UG) - 2021
- Biology
- recombinant technology
- During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
- NEET (UG) - 2021
- Biology
- recombinant technology
- During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out :
- NEET (UG) - 2021
- Biology
- recombinant technology
- Which of the following is not an application of $PCR$ (Polymerase Chain Reaction)?
- NEET (UG) - 2021
- Biology
- recombinant technology
View More Questions
Questions Asked in KCET exam
- The speed of sound in an ideal gas at a given temperature T is v. The rms speed of gas molecules at that temperature is vrms. The ratio of the velocities v and vrms for helium and oxygen gases are X and X' respectively. Then \(\frac{X}{X'}\) is equal to
- KCET - 2023
- Thermodynamics
- If \(p(\frac{1}{q}+\frac{1}{r}),q(\frac{1}{r}+\frac{1}{p}),r(\frac{1}{p}+\frac{1}{q})\) are in A.P., then p, q, r
- KCET - 2023
- Arithmetic Progression
- A stretched wire of a material whose Young's modulus Y = 2 × 1011 Nm-2 has Poisson's ratio of 0.25. Its lateral strain εl = 10-3. The elastic energy density of the wire is
- KCET - 2023
- mechanical properties of solids
- A metallic rod of length 1 m held along east-west direction is allowed to fall down freely. Given horizontal component of earth’s magnetic field BH = 3 × 10-5 T. The emf induced in the rod at an instant t = 2s after it is released is ( Take g = 10 ms-2 )
- KCET - 2023
- Faradays laws of induction
- A 60 W source emits monochromatic light of wavelength 662.5 nm. The number of photons emitted per second is
- KCET - 2023
- Quantum Physics
View More Questions
Concepts Used:
Recombinant Technology
Recombinant DNA technology is the process used for producing new genetic combinations by joining different genetic material (DNA) together and inserting them into host organisms from two different species or sources. These new combinations are of value to medicine, science, industry, and agriculture.
Process of Recombinant DNA Technology:
Step 1- Isolation of Genetic Material.
Step 2- Cutting the gene at the recognition sites.
Step 3- Amplifying the gene copies through Polymerase chain reaction ( PCR)
Step 4- Ligation of DNA Molecules.
Step 5- Insertion of Recombinant DNA into Host.
Application of Recombinant DNA Technology:
- In agricultural fields Recombinant DNA Technology plays a major role. It produces genetically-modified organisms such as flavor save tomatoes, golden rice rich in protein and lot more
- Recombinant DNA technology is also used to produce Insulin.
- ELISA is one kind of clinical diagnosis where recombinant DNA technology is used.
- Recombinant DNA technology prevents hereditary diseases through gene therapy and also detects the presence of HIV in a person.