Question

Which technique is not used for site directed mutagenesis?

• A. Oligonucleotide directed mutagenesis
• B. Cassette mutagenesis
• C. Physical mutagenesis
• D. SDM with PCR

Hint:

• Site-Directed Mutagenesis (SDM) Introduces specific, desired mutations at a defined location in a DNA sequence.
• Common SDM techniques: Oligonucleotide-directed (e.g., Kunkel method), PCR-based methods, cassette mutagenesis.
• Random Mutagenesis Introduces mutations non-specifically throughout a gene or genome. Can be induced by chemical mutagens (e.g., EMS, NTG) or physical mutagens (e.g., UV, X-rays).

Answer 1

The Correct Option is C

Site-directed mutagenesis (SDM) is a molecular biology method used to make specific and intentional changes to the DNA sequence of a gene. Common techniques for site-directed mutagenesis include:

• Oligonucleotide directed mutagenesis: This method utilizes short DNA fragments to induce specific nucleotide changes.
• Cassette mutagenesis: This involves replacing a segment of a DNA with a synthetic DNA (cassette) that contains mutations.
• SDM with PCR: Polymerase Chain Reaction (PCR) can amplify specific DNA sequences, introducing mutations through primers designed to include those mutations.

Physical mutagenesis, on the other hand, is not typically considered a site-directed mutagenesis technique. It involves using physical agents such as radiation (UV, X-rays) to create random mutations across the genome, rather than targeting specific DNA sequences.

Therefore, the technique that is not used for site-directed mutagenesis is: Physical mutagenesis