Question

A Thermal Cycler/Stabilizer is used for .........?

• A. Maintaining temperature
• B. DNA amplification (PCR)
• C. Protein synthesis
• D. RNA transcription

Hint:

Thermal cyclers are essential for PCR, which is a fundamental technique in genetics, forensics, and diagnostics.

Answer 1

The Correct Option is B

A thermal cycler, also known as a PCR machine, is widely used in molecular biology for DNA amplification through the Polymerase Chain Reaction (PCR). 
It involves: 
Denaturation: Heating the DNA to \(94-96^\circ \text{C}\) to separate the strands. 
Annealing: Cooling to \(50-65^\circ \text{C}\) to allow primers to bind to specific sequences. 
Extension: Raising the temperature to \(72^\circ \text{C}\) for DNA polymerase to synthesize new DNA strands. These cycles are repeated to exponentially amplify the DNA. 

Final Answer: \[ \boxed{\text{DNA amplification (PCR)}} \]