The LexA protein represses genes in the SOS response pathway. Under DNA damage (e.g.,UV light), LexA is cleaved, allowing transcription of genes it regulates. This engineering enables the production of β-galactosidase in response to LexA degradation caused by DNA damage.
Arrange the following in correct order in polymerase chain reaction cycle:
I Deannealing
II Denaturation
III Extension
IV Annealing