The work done to rotate the water molecule in an electric field is given by the formula:
\[
W = pE \left( 1 - \cos \theta \right)
\]
where:
- \( p \) is the dipole moment of the water molecule,
- \( E \) is the electric field,
- \( \theta \) is the angle of rotation (which is \( 180^\circ \), so \( \cos 180^\circ = -1 \)).
The work done to rotate the molecule is:
\[
W = pE \left( 1 - (-1) \right) = 2pE
\]
Therefore:
\[
p = \frac{W}{2E}
\]
Substitute the given values:
\[
p = \frac{5 \times 10^{-25}}{2 \times 2.5 \times 10^4} = \frac{5 \times 10^{-25}}{5 \times 10^4} = 10^{-29} \, \text{C} \cdot \text{m}
\]
Now, the dipole moment \( p \) is related to the separation between the charges \( d \) by the formula:
\[
p = qd
\]
where \( q \) is the charge on the water molecule and \( d \) is the separation between the charges.
For a water molecule, the charge is approximately the charge of an electron, \( q \approx 1.6 \times 10^{-19} \, \text{C} \).
Thus, we have:
\[
d = \frac{p}{q} = \frac{10^{-29}}{1.6 \times 10^{-19}} = 0.625 \times 10^{-10} \, \text{m}
\]
Thus, the approximate separation of the charges is \( 0.625 \times 10^{-10 \, \text{m}} \).