List of top Life Science Questions

With the addition of linking number (Lk) by +2 to a relaxed DNA consisting of Lk = 200, which of the following occurs?
What are the functions of topoisomerases?
What percentage of genes are found in the human genome?
Number of chromosomes present in Arabidopsis thaliana is:
Match List I with List II:
List IList II
(A) Histone erasers(III) Deacetylases
(B) Histone readers(IV) Bromodomain proteins
(C) Histone writers(I) Methyltransferase
(D) DNA unwinding(II) Topoisomerase
Choose the correct answer from the options given below:
Match List I with List II:
List I (Enzyme)List II (Reaction)
(A) DNase I(I) Produces single-stranded nicks in DNA
(B) RNase A(II) Degrades single-stranded RNA
(C) S1 nuclease(III) Degrades single-stranded DNA
(D) RNase H(IV) Cleaves the RNA strand of RNA-DNA hybrids
Choose the correct answer from the options given below:
Vertical gel electrophoresis is routinely used for the separation of:
Which is not a unit of radioactivity?
In the technique called centrifugation, the centrifugal field (G) is calculated as:
Isoelectric focusing allows separation of the proteins according to their:
Match List I with List II:
List IList II
(A) Western Blotting(II) Protein identification
(B) Southern Blotting(I) DNA identification
(C) Northern Blotting(IV) RNA identification
(D) Yeast two-hybrid system(III) Protein-protein interaction
Choose the correct answer from the options given below:
Arrange the given steps involved in polymerase chain reaction (PCR):
(A) Denaturation
(B) Annealing
(C) Extension
(D) Cycling
Choose the correct answer from the options given below:
Identify the correct sequence of the given enzymes involved in the TCA cycle:
(A) Aconitase
(B) Fumarase
(C) Malate dehydrogenase
(D) α-ketoglutarate dehydrogenase
Choose the correct answer from the options given below:
Which of the following is not a fluorescence dye?
The technique used to identify the specific DNA in bacterial colonies is:
Which metal is used for electron beam generation in scanning electron microscopy?
What is the role of sodium dodecyl sulfate (SDS) in protein electrophoresis?
(A) Add net negative charge to the protein
(B) Separate lipids on the basis of their molecular weight
(C) Modify native conformation of a protein
(D) Allows direct destaining of the proteins
Choose the correct answer from the options given below:
Match List I with List II:
List I (Vitamin)List II (Disorder)
(A) Vitamin-A(I) Night blindness
(B) Vitamin-D(II) Scurvy
(C) Vitamin-C(III) Fat-soluble vitamin
(D) Vitamin-K(IV) Blood clotting
Choose the correct answer from the options given below:
Identify the correct sequence of the given intermediates formed during glycolysis:
(A) Fructose 1,6-biphosphate
(B) Fructose 6 phosphate
(C) Lactate
(D) Phosphoenolpyruvate
Choose the correct answer from the options given below:
Which of the following enzyme is defective in galactosemia—a fatal genetic disorder in infants?
Which of the following enzyme is inhibited by citrate in Kreb's cycle?
Why does the presence of proline destabilize α-helix?
(A) Proline residue introduces a destabilizing kink in the α-helix
(B) In proline, the nitrogen atom is a part of a rigid ring, and rotation about N-C bond is not possible
(C) The nitrogen atom of a proline residue in a peptide linkage has no substituent hydrogen to participate in hydrogen bonds with other residues
(D) Proline residue works as a helix linker
Choose the correct answer from the options given below:
Which of the following are components of lipid rafts in plasma membrane?
(A) Cholesterol
(B) Sphingolipids
(C) Glycolipids
(D) Phospholipids
Choose the correct answer from the options given below:
Match List I with List II:
List I (Enzyme)List II (Function)
(A) Cytochrome oxidase(I) Electron transport system
(B) Topoisomerases(II) Change linking number
(C) Cohesins(III) DNA Replication
(D) PCNA(IV) Cell cycle
Choose the correct answer from the options given below:
The portion of transmembrane proteins that cross the lipid bilayer are usually: