List of top Biotechnology Tools and Techniques Questions asked in GATE BT

A DNA solution of 50 \(\mu\)g mL\(^{-1}\) gives absorbance of 1.0 at 260 nm. A 20 \(\mu\)L aliquot from a 50 \(\mu\)L purified plasmid solution is diluted to 1000 \(\mu\)L. The diluted solution gives absorbance 0.550 at 260 nm. The purified plasmid concentration in \(\mu\)g \(\mu\)L\(^{-1}\) (rounded to two decimals) is \(\underline{\hspace{1cm}}\).
The possible number of SalI restriction sites in a 9 kb double-stranded DNA with equal base proportion (nearest integer) is \(\underline{\hspace{1cm}}\).
Tertiary structure of a protein consisting of $\alpha$-helices and $\beta$-strands can be determined by:
Which of the following nucleus/nuclei is/are NMR active?
Which one of the following tools is used to compare all the possible six-open reading frames of a given nucleotide query sequence with all the available six-open reading frames of the nucleotide sequence database?
The enzyme which adds phosphate group to the free 5' terminus of a DNA sequence is
Which one of the following techniques/tools is NOT used for inserting a foreign gene into a cell?
CRISPR-Cas system is associated with:
Number of unrooted trees in a phylogeny of five sequences is
Which one of the following methods is used to test the significance of a predicted phylogeny?