Question

Write the role of ’ori’ and restriction site in the cloning vector pBR322.

Hint:

The ori site is essential for plasmid replication, and the restriction sites allow for the insertion of foreign DNA for genetic manipulation.

Answer 1

- ori (Origin of replication): The ori site in pBR322 allows the plasmid to replicate inside the host cell, ensuring that the vector is maintained and multiplied.
- Restriction site: The restriction sites in pBR322 are where specific restriction enzymes can cut the plasmid to insert foreign DNA for cloning purposes.