Question

Which one of the following is not a gene insertion technique into a eukaryotic cell

• A. Electroporation
• B. Iontophoresis
• C. Shot gun sequencing
• D. Microinjection

Answer 1

The Correct Option is B

To solve the question of which technique is not used for gene insertion into a eukaryotic cell, we need to understand the purpose and method of each technique listed in the options.

1. Electroporation: This technique uses an electric field to increase the permeability of the cell membrane, allowing foreign DNA to enter the cell. It is a common and effective method for gene insertion into eukaryotic cells.
2. Iontophoresis: This technique involves the transport of ions across the skin or tissues using a small electric current. It is typically used in drug delivery systems and not for gene insertion into cells. Thus, it is not used for inserting genes into eukaryotic cells.
3. Shotgun Sequencing: This technique is used for sequencing DNA, not for inserting it into cells. It involves randomly breaking up DNA sequences and then reassembling the sequences to map the original DNA. It isn't a method for gene insertion.
4. Microinjection: This is a precise method where a fine glass needle is used to directly inject DNA into the nucleus of a eukaryotic cell. It is a common technique for gene insertion.

Based on the explanations above, the technique that does not pertain to the insertion of genes into eukaryotic cells is Iontophoresis. While Shotgun Sequencing is not a gene insertion technique, it is also not designed for this purpose, similar to Iontophoresis.

Thus, the most relevant incorrect option, according to the context of gene insertion, and given the options, is Iontophoresis. Therefore, the correct answer is Iontophoresis.