RuBisCO (Ribulose-1,5-bisphosphate carboxylase/oxygenase) is the key enzyme in the Calvin cycle (dark reactions of photosynthesis). It catalyzes the fixation of CO2 by adding it to RuBP (ribulose-1,5-bisphosphate). While it can also react with oxygen (photorespiration), its affinity for CO2 is higher under normal conditions. It is not involved in the photolysis of water, which occurs in the light-dependent reactions.