Question:
Which of the following is used as spray reagent for detection of steroids?
Which of the following is used as spray reagent for detection of steroids?
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Think of "antimony" and "chloride" when you need to spot steroids on a TLC plate.
Updated On: May 6, 2025
- \( \text{Antimony trichloride} \)
- \( \text{Bromothymol blue} \)
- \( \text{Diphenyl carbazone} \)
- \( \text{Mercuric nitrate} \)
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The Correct Option is A
Solution and Explanation
Antimony trichloride (\( \text{SbCl}_3 \)) in chloroform or acetic anhydride is a commonly used spray reagent for the detection of steroids on thin-layer chromatography (TLC) plates. Upon heating after spraying, steroids react with antimony trichloride to produce colored spots, allowing for their visualization and identification.
Bromothymol blue is a pH indicator. Diphenyl carbazone is used as an indicator for mercury and other metal ions. Mercuric nitrate is a reagent used in various chemical tests but is not a common spray reagent for the general detection of steroids on TLC.
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