Question

Which of the following is not an application of $PCR$ (Polymerase Chain Reaction)?

• A. Molecular diagnosis
• B. Gene amplification
• C. Purification of isolated protein
• D. Detection of gene mutation

Answer 1

The Correct Option is C

Polymerase Chain Reaction (PCR) is a revolutionary technique in molecular biology that allows for the amplification of a specific DNA segment. It has various applications, particularly in fields related to genetics and molecular biology. Let's evaluate the given options to determine which one is not an application of PCR.

1. Molecular diagnosis: PCR is widely used in molecular diagnosis to identify pathogens and genetic disorders by amplifying specific DNA sequences related to the organism or condition. Hence, this is an application of PCR.
2. Gene amplification: The primary purpose of PCR is to amplify or make multiple copies of a particular gene or DNA segment. Therefore, gene amplification is indeed a principal application of PCR.
3. Purification of isolated protein: PCR is not directly used for the purification of proteins. Protein purification typically involves techniques like chromatography or electrophoresis, which separate proteins based on properties such as size and charge. Thus, this is not an application of PCR.
4. Detection of gene mutation: PCR is frequently employed to detect mutations in genes by comparing the amplified genes with known sequences. Variations can indicate mutations. Thus, this is an application of PCR.

Based on the explanations above, the option that is not an application of PCR is the purification of isolated protein.