Question:

Which of the following is not a characteristic of $pBR322$ vector?

Updated On: Aug 1, 2022
  • It was the first artificial cloning vector constructed in $1977$ by Boliver and Rodriguez
  • It is the most widely used, versatile and easily manipulated vector
  • It has two antibiotic resistance genes $tet^R$ and $amp^R$
  • It does not have restriction site for Sal I
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The Correct Option is D

Solution and Explanation

pBR322 has a restriction site for Sal I.
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Concepts Used:

Biotechnology

Biotechnology is a vast field of biology that involves the creation, production, and modification of useful products for human welfare using both the technology and the execution of living organisms and their components. An agricultural engineer, Karoly Ereky, coined the word "biotechnology" in 1919, achieving him the title of "Father of Biotechnology."

Principles of Biotechnology:

Among many, the two core techniques that entitled the birth of modern biotechnology are :

  1. Genetic Engineering is one of the methods of direct modifications of an organism's genome (i.e., DNA and RNA). It requires the introduction of new genes into host species to escalate function or trait, hence altering the host organism's phenotype.
  2. Bioprocess Engineering is one of the methods of maintaining sterile conditions to facilitate the growth of large quantities of desired microbes and other eukaryotic cells for the development of new or modified biotechnological products such as enzymes, vaccines, antibiotics, and other biotechnological products.