Question

Which of the following does not have a negative effect on PFK?

• A. ATP
• B. Citrate
• C. pH
• D. AMP

Hint:

PFK is activated by AMP and inhibited by ATP and citrate, which helps regulate glycolysis based on the energy status of the cell.

Answer 1

The Correct Option is D

- ATP, citrate, and low pH all inhibit phosphofructokinase (PFK), which is a key enzyme in glycolysis. These factors indicate high energy levels or unfavorable conditions for glycolysis.
- AMP, however, stimulates PFK as it indicates low energy levels in the cell, signaling the need for increased glycolysis to produce more ATP.

Conclusion: AMP stimulates PFK activity, so option (d) is the correct answer.