Somatic hybridization involves the fusion of protoplasts (cells without a cell wall) from two different plant varieties. This technique is used to create hybrid plants with desired traits.
Step 1: Totipotency refers to the ability of a single plant cell to develop into a whole plant. This property is unique to certain plant cells and allows for plant regeneration through methods like tissue culture.
Step 2: In totipotent cells, all genetic information required for the development of the entire plant is retained, which allows these cells to differentiate into all types of plant tissues, leading to the formation of a complete plant.
Thus, the ability to generate a whole plant from a single cell is called totipotency.
List-I (Hybridization) | List-II (Orientation in Space) |
---|---|
(A) sp3 | (I) Trigonal bipyramidal |
(B) dsp2 | (II) Octahedral |
(C) sp3d | (III) Tetrahedral |
(D) sp3d2 | (IV) Square planar |
LIST I (Instrumentation/Technique) | LIST II (Useful for) | ||
A. | Hybridization | I. | Purification of biomolecules |
B. | Confocal scanning microscope | II. | Separation of proteins |
C. | Western Blotting | III. | Three-dimensional image |
D. | Chromatography | IV. | Location of complementary DNA |
List I (Spectral Lines of Hydrogen for transitions from) | List II (Wavelength (nm)) | ||
A. | n2 = 3 to n1 = 2 | I. | 410.2 |
B. | n2 = 4 to n1 = 2 | II. | 434.1 |
C. | n2 = 5 to n1 = 2 | III. | 656.3 |
D. | n2 = 6 to n1 = 2 | IV. | 486.1 |
The following diagram shown restriction sites in E. coli cloning vector pBR322. Find the role of ‘X’ and ‘Y’gens :