Question

Which centrifugation depends on buoyant densities?

• A. Isopycnic centrifugation
• B. Gradient centrifugation
• C. Density gradient centrifugation
• D. Differential centrifugation

Answer 1

The Correct Option is A

Step 1: Understanding isopycnic centrifugation. - Isopycnic centrifugation is a technique where particles migrate to a position where their buoyant density matches the surrounding medium. - This method allows separation based solely on density differences. 

Step 2: Explanation of incorrect options. - (B) Gradient centrifugation: A general term, but it does not specifically refer to buoyant density-based separation. - (C) Density gradient centrifugation: A broader term, but it includes both rate-zonal and isopycnic centrifugation. - (D) Differential centrifugation: Separates based on size and mass, not buoyant density. 

Step 3: Selecting the correct option. Since isopycnic centrifugation separates particles based on buoyant density, the correct answer is (A) Isopycnic centrifugation.