Question:
Where does EcoRI cut the palindrome? Write the events followed thereafter to form a recombinant DNA.
Where does EcoRI cut the palindrome? Write the events followed thereafter to form a recombinant DNA.
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EcoRI is a restriction enzyme widely used in genetic engineering for creating recombinant DNA molecules.
Updated On: Jan 20, 2025
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Solution and Explanation
EcoRI cuts between G and A in the sequence, generating sticky ends.
Events that follow:
The sticky ends of vector DNA and foreign DNA pair through complementary base pairing.
DNA ligase seals the nicks, forming recombinant DNA.
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