Question

Where does EcoRI cut the palindrome? Write the events followed thereafter to form a recombinant DNA.

Hint:

EcoRI is a restriction enzyme widely used in genetic engineering for creating recombinant DNA molecules.

Answer 1

EcoRI cuts between G and A in the sequence, generating sticky ends. Events that follow: The sticky ends of vector DNA and foreign DNA pair through complementary base pairing. DNA ligase seals the nicks, forming recombinant DNA.