Question:

Visualization of viral particles by Electron microscopy requires at least:

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Electron microscopy is highly sensitive but still requires a threshold concentration of viral particles ($10^{5}$–$10^{6}$/ml).
Updated On: Sep 26, 2025
  • $10$ to $10^{2}$ Particles/ml
  • $10^{3}$ to $10^{4}$ Particles/ml
  • $10^{5}$ to $10^{6}$ Particles/ml
  • $10^{12}$ to $10^{16}$ Particles/ml
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The Correct Option is C

Solution and Explanation

Step 1: Principle of electron microscopy.
Electron microscopy is a high-resolution imaging technique that requires a sufficient concentration of viral particles in a sample to produce visible images.
Unlike light microscopy, electron microscopy depends on electron scattering, and the viral load must be high enough to form clear contrast.
Step 2: Minimum concentration required.
For visualization, approximately $10^{5}$ to $10^{6}$ particles per milliliter are needed.
If the concentration is lower, the viral particles become too sparse to detect clearly.
Step 3: Analysis of options.
- (A) $10$ to $10^{2}$ Particles/ml: Too low, visualization not possible.
- (B) $10^{3}$ to $10^{4}$ Particles/ml: Still below the required concentration.
- (C) $10^{5}$ to $10^{6}$ Particles/ml: Correct, this is the threshold range for reliable visualization.
- (D) $10^{12}$ to $10^{16}$ Particles/ml: Extremely high and unrealistic concentration.
Step 4: Conclusion.
At least $10^{5}$ to $10^{6}$ particles/ml are required for visualization of viruses by electron microscopy.
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