Step 1: Recall function of AV valves.
Atrioventricular (AV) valves (tricuspid and mitral) regulate blood flow between atria and ventricles, preventing backflow.
Step 2: Sequence of operation.
1. Blood returning to atria increases pressure, forcing AV valves open (D).
2. Once open, valve flaps hang limply into ventricles (E).
3. Atria contract, pushing more blood into ventricles (A).
4. Ventricles then contract, pushing blood against AV valve flaps (C).
5. Chordae tendinae tighten to keep valve flaps closed and prevent backflow (B).
Step 3: Analysis of options.
The correct sequence is D $\rightarrow$ E $\rightarrow$ A $\rightarrow$ C $\rightarrow$ B.
Step 4: Importance.
This sequence ensures unidirectional blood flow — atria to ventricles, then ventricles to arteries, without regurgitation.