Question

To obtain the best results in any of the quantitative TLC methods, the spots being used should have \( R_f \) values between _______.

• A. 0.01 to 0.3
• B. 0.3 to 0.7
• C. 0.7 to 1.0
• D. > 1.0

Hint:

Choose solvents that give clear \( R_f \) values in the 0.3–0.7 range for best TLC results.

Answer 1

The Correct Option is B

For optimal separation and quantification in TLC, the \( R_f \) values of the spots should ideally lie between 0.3 and 0.7. This range provides a clear distance between origin and solvent front, allowing precise measurement and minimizing overlap.