The Ti (tumor-inducing) plasmid is a large plasmid found in the bacterium Agrobacterium tumefaciens. This plasmid is responsible for transferring a segment of its DNA into plant cells, causing the formation of crown gall tumors. The Ti plasmid is widely utilized in plant genetic engineering to introduce new genes into plants.
Researchers exploit the Ti plasmid's natural ability to transfer DNA to manipulate plant genomes. By replacing the tumor-inducing genes with desired genes, scientists can create genetically modified plants with beneficial traits, such as pest resistance or increased yield.
Annealing process of PCR is performed at \(\_\_\_\) temperature.