Question

The technique of ELISA is based on which of the following?

• A. DNA-RNA interaction
• B. DNA-protein interaction
• C. Antigen-antibody interaction
• D. RNA-protein interaction

Hint:

Remember: ELISA = Antigen–Antibody test, commonly used in HIV, pregnancy, and viral infection detection.

Answer 1

The Correct Option is C

Step 1: Define ELISA.
ELISA = Enzyme Linked Immunosorbent Assay, a diagnostic tool to detect presence of antigen or antibody in a sample.
Step 2: Principle.
It works on the principle of a specific antigen-antibody reaction. An enzyme is linked to either antigen or antibody, producing a color change when the substrate is added.
Step 3: Checking options.
- DNA-RNA → Used in hybridization assays, not ELISA.
- DNA-protein → Not applicable here.
- Antigen-antibody → Correct, principle of ELISA.
- RNA-protein → Incorrect.
Step 4: Conclusion.
Thus, ELISA is based on antigen-antibody interaction.