Question

The production of haploids by anther and pollen culture was first demonstrated by -

• A. Maheswari and Guha
• B. Ravi and Chan
• C. Kasha and Kao
• D. Clausen and Cameron

Hint:

For competitive exams in biology and biotechnology, it's crucial to remember the names of key scientists associated with major discoveries, such as Mendel (genetics), Watson and Crick (DNA structure), and in this case, Maheswari and Guha (anther culture).

Answer 1

The Correct Option is A

Step 1: Understanding the Concept:
This question is about a significant historical discovery in the field of plant tissue culture. It asks to identify the scientists who first successfully produced haploid plants using anther and pollen culture techniques.
Step 2: Detailed Explanation:
The groundbreaking work on producing haploids through in vitro culture of anthers was performed by two Indian scientists, Sipra Guha and Satish C. Maheshwari.
In 1964, they reported the direct development of embryos from microspores of Datura innoxia (thorn apple) through the culture of its excised anthers.
This discovery was a milestone in plant biotechnology as it opened up a rapid method for producing homozygous diploid lines through chromosome doubling of haploids, significantly speeding up breeding programs.
The other options list scientists known for other contributions but not this specific discovery.
Step 3: Final Answer:
The first demonstration of haploid production from anther culture was by Maheswari and Guha.