Question

The presence of antibody in infected patient's serum can be detected by

• A. ELISPOT
• B. PCR
• C. Northern blot
• D. Western blot

Hint:

Use Western blot when you need to confirm the presence of specific proteins or antibodies in a serum or tissue extract, especially for diagnostic purposes like HIV detection.

Answer 1

The Correct Option is D

Step 1: Understanding Western blot technique.
Western blotting is a widely used analytical technique to detect specific proteins (including antibodies) in a sample. It uses a combination of gel electrophoresis and specific antibody binding.
Step 2: Application to antibodies.
In infected patients, antibodies generated in response to pathogens can be identified by using antigen-coated membranes in Western blot, followed by enzyme-linked secondary antibody detection.
Step 3: Eliminate other options.
ELISPOT is used to measure cytokine secretion or specific cell responses, not circulating antibodies.
PCR detects DNA or RNA, not proteins.
Northern blot is for RNA detection.