Step 1: Understanding the exponential growth formula.
The exponential growth of bacteria is given by the formula:
\[
N(t) = N_0 \times 2^{t/T},
\]
where \( N(t) \) is the population at time \( t \), \( N_0 \) is the initial population, and \( T \) is the generation time.
Step 2: Substituting the known values.
Given that the initial population is \( 10^6 \), the final population is \( 4 \times 10^6 \), and the generation time is 20 minutes, we need to solve for \( t \) when:
\[
4 \times 10^6 = 10^6 \times 2^{t/20}.
\]
Simplifying:
\[
4 = 2^{t/20}.
\]
Step 3: Solving for \( t \).
Taking the logarithm of both sides:
\[
\log_2(4) = \frac{t}{20}.
\]
Since \( \log_2(4) = 2 \), we have:
\[
2 = \frac{t}{20}.
\]
Solving for \( t \):
\[
t = 40 \, \text{minutes}.
\]
Step 4: Conclusion.
The correct answer is \( \boxed{40} \, \text{minutes} \).




Which of the following microbes is NOT involved in the preparation of household products?
A. \(\textit{Aspergillus niger}\)
B. \(\textit{Lactobacillus}\)
C. \(\textit{Trichoderma polysporum}\)
D. \(\textit{Saccharomyces cerevisiae}\)
E. \(\textit{Propionibacterium sharmanii}\)
Identify the taxa that constitute a paraphyletic group in the given phylogenetic tree.
The vector, shown in the figure, has promoter and RBS sequences in the 300 bp region between the restriction sites for enzymes X and Y. There are no other sites for X and Y in the vector. The promoter is directed towards the Y site. The insert containing only an ORF provides 3 fragments after digestion with both enzymes X and Y. The ORF is cloned in the correct orientation in the vector using the single restriction enzyme Y. The size of the largest fragment of the recombinant plasmid expressing the ORF upon digestion with enzyme X is ........... bp. (answer in integer) 