Question

The component of bacteria that retains the crystal violet stain during Gram-staining is

• A. O-antigen
• B. Lipopolysaccharide
• C. Peptidoglycan
• D. Cytoplasmic membrane

Answer 1

The Correct Option is C

 The stain is retained by the peptidoglycan layer within the cell wall of the bacteria. They are classified into gram positive and gram negative. Gram positive has a thick layer of peptidoglycan, that prevents the stain from washing away. Gram negative on the other hand, has a thin layer of peptidoglycan.