We are given two straight lines in parametric form:
\[
\overrightarrow{r_1} = (4i - k) + t(2i + j - 2k), \quad t \in \mathbb{R},
\]
and
\[
\overrightarrow{r_2} = (i - j + 2k) + s(2i - 2j + k), \quad s \in \mathbb{R}.
\]
Step 1: Find the direction ratios of the two lines.
For \( \overrightarrow{r_1} \), the direction ratios are the coefficients of \( t \), which are:
\[
\overrightarrow{a_1} = 2i + j - 2k.
\]
For \( \overrightarrow{r_2} \), the direction ratios are the coefficients of \( s \), which are:
\[
\overrightarrow{a_2} = 2i - 2j + k.
\]
Step 2: Use the formula for the angle between two vectors:
\[
\cos\theta = \frac{\overrightarrow{a_1} \cdot \overrightarrow{a_2}}{|\overrightarrow{a_1}| |\overrightarrow{a_2}|}.
\]
The dot product \( \overrightarrow{a_1} \cdot \overrightarrow{a_2} \) is:
\[
\overrightarrow{a_1} \cdot \overrightarrow{a_2} = (2)(2) + (1)(-2) + (-2)(1) = 4 - 2 - 2 = 0.
\]
Since the dot product is 0, the vectors are perpendicular, which means the angle \( \theta \) between the two vectors is:
\[
\theta = \frac{\pi}{2}.
\]
Thus, the angle between the two lines is \( \frac{\pi}{2} \), and the correct answer is option (E).