Question

State the advantage of using thermostable DNA polymerase.

Hint:

Thermostable DNA polymerases such as Taq polymerase allow for continuous amplification of DNA without the need for enzyme replenishment at each cycle, making PCR highly efficient.

Answer 1

Advantage of using thermostable DNA polymerase:
- The main advantage of using thermostable DNA polymerase, like \textit{Taq polymerase}, is its ability to withstand the high temperatures required during the denaturation step of PCR (usually around 94-98°C).
- This allows the enzyme to remain active throughout the PCR process, eliminating the need to add fresh enzyme after each cycle, thus making the process more efficient and time-saving.