Question

....... should enter the cell in the case of activation of reporter gene for two hybrid system.

• A. Bait protein
• B. Prey protein
• C. Both bait and prey protein
• D. Either one of them

Hint:

In a two-hybrid system, the interaction of both bait and prey proteins is required to activate the reporter gene, enabling detection of protein-protein interactions.

Answer 1

The Correct Option is C

Step 1: Understanding the two-hybrid system.
In the two-hybrid system, the goal is to test for protein-protein interactions. The bait protein is fused to a DNA binding domain, and the prey protein is fused to an activation domain. Both proteins are expressed in the cell to assess if they interact, activating the reporter gene.
Step 2: Analyzing the options.
(1) Bait protein: This alone would not activate the reporter gene. It requires interaction with the prey protein.
(2) Prey protein: This alone would not activate the reporter gene. It needs the bait protein to interact.
(3) Both bait and prey protein: Correct — Both proteins need to be present in the cell to test for interaction, which will activate the reporter gene.
(4) Either one of them: This is incorrect because both proteins need to interact to activate the reporter gene.
Step 3: Conclusion.
The correct answer is (3) Both bait and prey protein, as both are necessary for activating the reporter gene in a two-hybrid system.