Question

Select the correct statement from the following biotechnological procedures:

• A. The polymerase enzyme joins the gene of interest and the vector DNA.
• B. Gel electrophoresis is used for amplification of a DNA segment.
• C. PCR is used for isolation and separation of the gene of interest.
• D. Plasmid DNA acts as vector to transfer the piece of DNA attached to it.

Hint:

Plasmids are widely used as vectors in genetic engineering because they can replicate independently in a host cell and carry foreign DNA.

Answer 1

The Correct Option is D

Step 1: In biotechnology, a vector is a vehicle used to transfer a gene of interest into a host cell.
Step 2: Plasmid DNA is commonly used as a vector in genetic engineering to carry and transfer the desired DNA fragment into a host organism.
Step 3: The other options are incorrect: polymerase does not join DNA (ligase does), gel electrophoresis separates DNA, and PCR amplifies DNA, not isolates it.
Thus, the correct statement is that plasmid DNA acts as a vector to transfer the piece of DNA attached to it.