Question

Select the correct statement from the following :

• A. DNA from one organism will not band to DNA from other organism.
• B. Genetic engineering works only on animals and not yet successfully used on plants.
• C. There are no risk factors associated with r-DNA technology.
• D. The first step in PCR in heating which is used to seperate both the strands of gene of interest.

Answer 1

The Correct Option is D

The correct answer is (D) : The first step in PCR in heating which is used to seperate both the strands of gene of interest.

Answer 2

The correct answer is: (D) The first step in PCR is heating, which is used to separate both the strands of the gene of interest.

Polymerase Chain Reaction (PCR) is a powerful technique used to amplify specific DNA sequences. The process of PCR consists of several steps, each critical for the successful amplification of the DNA. These steps include:

• Denaturation: The first step in PCR is heating the reaction mixture to around 94-98°C. This heat causes the DNA double helix to separate, or "denature," into two single strands. This step is crucial because it allows the primers to bind to the single-stranded DNA templates in the next step.
• Annealing: The temperature is then lowered to allow primers to bind to their complementary sequences on the single-stranded DNA templates.
• Extension: The temperature is raised again to allow the DNA polymerase enzyme to synthesize the new DNA strand, starting from the primer and using the original strand as a template.

Thus, the first step in PCR is indeed heating, which is used to separate the DNA strands, and this makes (D) the correct answer.