Satellite RNAs are present in some
- Priom
- Bacteriophages
- Plant viruses
- Viroids
The Correct Option is C
Solution and Explanation
Top Questions on DNA fingerprinting
- In DNA fingerprinting, which of the following techniques is used to amplify specific regions of DNA for analysis?
- MHT CET - 2024
- Biology
- DNA fingerprinting
- Arrange the given steps of DNA finger printing technique in correct sequence.
A. Isolation of DNA and its digestion by restriction and endonuclease
B. Seperation of DNA fragments by electrophoresis
C. Hybridization with labelled VNTR probe and autoradiography
D. Blotting of seperated DNA fragments to synthetic membrane
Choose the correct answer from the options given below :- CUET (UG) - 2023
- Biology
- DNA fingerprinting
- The steps of DNA fingerprinting are given below. Arrange them in a correct sequence.
(A)Separation of DNA fragments by electrophoresis
(B)Hybridization using labelled VNTR probe
(C)Isolation and digestion of DNA
(D)Detection of hybridized DNA fragments by autoradiography
(E)Transferring Of separated DNA fragments to synthetic membranes
Choose the correct answer from the options given below :- CUET (UG) - 2023
- Biology
- DNA fingerprinting
Which aspect forms the basis of DNA finger- printing?
- KCET - 2022
- Biology
- DNA fingerprinting
- DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as :
- NEET (UG) - 2021
- Biology
- DNA fingerprinting
Questions Asked in NEET exam
- A microscope has an objective of focal length \(f_o = 2\) cm and an eyepiece of focal length \(f_e = 4\) cm. The tube length of the microscope is \(L = 40\) cm. If the distance of distinct vision of eye is \(D = 25\) cm, the magnification in the microscope is:
- NEET (UG) - 2025
- Optical Instruments
A sphere of radius R is cut from a larger solid sphere of radius 2R as shown in the figure. The ratio of the moment of inertia of the smaller sphere to that of the rest part of the sphere about the Y-axis is :
- NEET (UG) - 2025
- Moment Of Inertia
AB is a part of an electrical circuit (see figure). The potential difference \(V_A - V_B\), at the instant when current \(i = 2\) A and is increasing at a rate of 1 amp/second is:
- NEET (UG) - 2025
- Electromagnetic Induction and Inductance
- Twins are born to a family that lives next door to you. The twins are a boy and a girl. Which of the following must be true?
- NEET (UG) - 2025
- Genetics
Three identical heat conducting rods are connected in series as shown in the figure. The rods on the sides have thermal conductivity 2K while that in the middle has thermal conductivity K. The left end of the combination is maintained at temperature 3T and the right end at T. The rods are thermally insulated from outside. In steady state, temperature at the left junction is \(T_1\) and that at the right junction is \(T_2\). The ratio \(T_1 / T_2\) is
- NEET (UG) - 2025
- Conductivity-thermal and electrical
Concepts Used:
DNA Fingerprinting
DNA fingerprinting, also called DNA typing, DNA profiling, genetic fingerprinting, genotyping, or identity testing, in genetics, method of isolating and identifying variable elements within the base-pair sequence of DNA.
The procedure of DNA Fingerprinting:
The procedure for creating a DNA fingerprint consists of first obtaining a sample of cells, such as skin, hair, or blood cells, which contain DNA. The DNA is extracted from the cells and purified. In Jeffreys’s original approach, which was based on restriction fragment length polymorphism (RFLP) technology, the DNA was then cut at specific points along the strand with proteins known as restriction enzymes. The enzymes produced fragments of varying lengths that were sorted by placing them on a gel and then subjecting the gel to an electric current (electrophoresis): the shorter the fragment, the more quickly it moved toward the positive pole (anode). The sorted double-stranded DNA fragments were then subjected to a blotting technique in which they were split into single strands and transferred to a nylon sheet. The fragments underwent autoradiography in which they were exposed to DNA probes—pieces of synthetic DNA that were made radioactive and that bound to the minisatellites. A piece of X-ray film was then exposed to the fragments, and a dark mark was produced at any point where a radioactive probe had become attached. The resultant pattern of marks could then be analyzed.