DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as :
- Yellow bands
- Bright orange bands
- Dark red bands
- Bright blue bands
The Correct Option is B
Solution and Explanation
So, the correct option is (B): Bright orange bands.
Top Questions on DNA fingerprinting
- Arrange the given steps of DNA finger printing technique in correct sequence.
A. Isolation of DNA and its digestion by restriction and endonuclease
B. Seperation of DNA fragments by electrophoresis
C. Hybridization with labelled VNTR probe and autoradiography
D. Blotting of seperated DNA fragments to synthetic membrane
Choose the correct answer from the options given below :- CUET (UG) - 2023
- Biology
- DNA fingerprinting
- The steps of DNA fingerprinting are given below. Arrange them in a correct sequence.
(A)Separation of DNA fragments by electrophoresis
(B)Hybridization using labelled VNTR probe
(C)Isolation and digestion of DNA
(D)Detection of hybridized DNA fragments by autoradiography
(E)Transferring Of separated DNA fragments to synthetic membranes
Choose the correct answer from the options given below :- CUET (UG) - 2023
- Biology
- DNA fingerprinting
Which aspect forms the basis of DNA finger- printing?
- KCET - 2022
- Biology
- DNA fingerprinting
- DNA fingerprinting involves identifying differences in some specific regions in DNA sequence, called as:
- NEET (UG) - 2021
- Biology
- DNA fingerprinting
Complete the flow chart on central dogma.
- NEET (UG) - 2021
- Biology
- DNA fingerprinting
Questions Asked in NEET exam
- Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R.
Assertion A : The potential (V) at any axial point, at 2 m distance(r) from the centre of the dipole of dipole moment vector \(\vec{P}\) of magnitude, 4 × 10-6 C m, is ± 9 × 103 V.
(Take \(\frac{1}{4\pi\epsilon_0}=9\times10^9\) SI units)
Reason R : \(V=±\frac{2P}{4\pi \epsilon_0r^2}\), where r is the distance of any axial point, situated at 2 m from the centre of the dipole.
In the light of the above statements, choose the correct answer from the options given below :- NEET (UG) - 2024
- Magnetic Field
- Given below are two statements:
Statement I: Mitochondria and chloroplasts both double membranes bound organelles.
Statement II: Inner membrane of mitochondria is relatively less permeable, as compared chloroplast. I
In the light of the above statements, choose the mis appropriate answer from the options given below- NEET (UG) - 2024
- Cell structure and organelles
- Match List I with List II:Choose the correct answer from the options given below:
List I
List II
A Mesozoic Era I Lower invertebrates B Proterozoic Era II Fish & Amphibia C Cenozoic Era III Birds & Reptiles D Paleozoic Era IV Mammals - NEET (UG) - 2024
- Origin Of Life
- A parallel plate capacitor is charged by connecting it to a battery through a resistor. If I is the current in the circuit, then in the gap between the plates :
- NEET (UG) - 2024
- Capacitors and Capacitance
- The terminal voltage of the battery, whose emf is\(10V\) and internal resistance \(1\), when connected through an external resistance of \(42\) as shown in the figure is:
- NEET (UG) - 2024
- Resistance and Resistivity
Concepts Used:
DNA Fingerprinting
DNA fingerprinting, also called DNA typing, DNA profiling, genetic fingerprinting, genotyping, or identity testing, in genetics, method of isolating and identifying variable elements within the base-pair sequence of DNA.
The procedure of DNA Fingerprinting:
The procedure for creating a DNA fingerprint consists of first obtaining a sample of cells, such as skin, hair, or blood cells, which contain DNA. The DNA is extracted from the cells and purified. In Jeffreys’s original approach, which was based on restriction fragment length polymorphism (RFLP) technology, the DNA was then cut at specific points along the strand with proteins known as restriction enzymes. The enzymes produced fragments of varying lengths that were sorted by placing them on a gel and then subjecting the gel to an electric current (electrophoresis): the shorter the fragment, the more quickly it moved toward the positive pole (anode). The sorted double-stranded DNA fragments were then subjected to a blotting technique in which they were split into single strands and transferred to a nylon sheet. The fragments underwent autoradiography in which they were exposed to DNA probes—pieces of synthetic DNA that were made radioactive and that bound to the minisatellites. A piece of X-ray film was then exposed to the fragments, and a dark mark was produced at any point where a radioactive probe had become attached. The resultant pattern of marks could then be analyzed.