To answer the question about restriction enzymes, let's explore the relevant concepts:
Restriction enzymes, also known as restriction endonucleases, are proteins that cut DNA at specific sequences. They are essential tools in molecular biology, especially in cloning and analysis of genes. Understanding their classification helps in identifying their specific functions:
Given the definitions, the correct answer is neoschizomers because they are enzymes that recognize the same nucleotide sequence but cleave at different positions when compared to their isoschizomers.
Therefore, the concept and distinguishing feature of neoschizomers fits the description in the question, leading to the correct answer choice.
Identify the taxa that constitute a paraphyletic group in the given phylogenetic tree.
The vector, shown in the figure, has promoter and RBS sequences in the 300 bp region between the restriction sites for enzymes X and Y. There are no other sites for X and Y in the vector. The promoter is directed towards the Y site. The insert containing only an ORF provides 3 fragments after digestion with both enzymes X and Y. The ORF is cloned in the correct orientation in the vector using the single restriction enzyme Y. The size of the largest fragment of the recombinant plasmid expressing the ORF upon digestion with enzyme X is ........... bp. (answer in integer) 