Restriction endonuclease:
- cuts the DNA molecule randomly
- cuts the DNA molecule at specific sites
- restricts the synthesis of DNA inside the nucleus
- synthesizes DNA
The Correct Option is B
Solution and Explanation
Restriction endonucleases, also known as restriction enzymes, are enzymes that cleave DNA at specific recognition sequences or sites. These recognition sequences are typically palindromic sequences, and restriction enzymes cut the DNA at or near these specific sites. This is a fundamental tool in molecular biology and genetic engineering for manipulating DNA molecules.
So, the correct option is (B): cuts the DNA molecule at specific sites
Top Questions on Biotechnology
The vector, shown in the figure, has promoter and RBS sequences in the 300 bp region between the restriction sites for enzymes X and Y. There are no other sites for X and Y in the vector. The promoter is directed towards the Y site. The insert containing only an ORF provides 3 fragments after digestion with both enzymes X and Y. The ORF is cloned in the correct orientation in the vector using the single restriction enzyme Y. The size of the largest fragment of the recombinant plasmid expressing the ORF upon digestion with enzyme X is ........... bp. (answer in integer)
- IIT JAM BT - 2025
- Biotechnology
- Biotechnology
- Which of the following condition(s) lead(s) to reduced affinity of O2 for human hemoglobin?
- IIT JAM BT - 2025
- Biotechnology
- Biotechnology
Which of the following is/are CORRECT for the two molecules shown?
- IIT JAM BT - 2025
- Biotechnology
- Biotechnology
- Match the pair of organisms in Group I with their community interaction pattern in Group II. \[ \begin{array}{c|c} \text{Group I} & \text{Group II} \\ \hline P:\; \text{Cattle egrets – African buffalo} & 3:\; \text{Commensalism} \\ Q:\; \text{Lynx – Fox} & 1:\; \text{Competition} \\ R:\; \text{Acacia tree – Pugnacious ants} & 4:\; \text{Mutualism} \\ S:\; \text{Leopard – Antelope} & 2:\; \text{Predation} \\ \end{array} \]
- IIT JAM BT - 2025
- Biotechnology
- Biotechnology
- In a reaction \( A + B \to C \), the initial rate of formation of C at 25°C was measured for different initial concentrations of A and B as given. The overall order of the reaction with respect to both A and B is ................. (answer in integer)
% Requires: \usepackage{graphicx} \begin{table}[h] \centering \begin{tabular}{|c|c|c|c|} \hline Experiment & Initial [A] & Initial [B] & Initial rate of formation of C
& (mol L\textsuperscript{-1}) & (mol L\textsuperscript{-1}) & (mol L\textsuperscript{-1} s\textsuperscript{-1})
\hline 1 & 0.4 & 0.3 & 0.078
2 & 0.8 & 0.3 & 0.312
3 & 0.4 & 0.6 & 0.156
4 & 0.8 & 0.6 & 0.624
\hline \end{tabular} \caption{Experimental data showing initial concentrations and rates of formation.} \label{tab:experimental_data} \end{table} \flushleft \newpage- IIT JAM BT - 2025
- Biotechnology
- Biotechnology
Questions Asked in NEET exam
The current passing through the battery in the given circuit, is:
- NEET (UG) - 2025
- Current electricity
A bob of heavy mass \(m\) is suspended by a light string of length \(l\). The bob is given a horizontal velocity \(v_0\) as shown in figure. If the string gets slack at some point P making an angle \( \theta \) from the horizontal, the ratio of the speed \(v\) of the bob at point P to its initial speed \(v_0\) is :
- NEET (UG) - 2025
- Pendulums
- Two cities X and Y are connected by a regular bus service with a bus leaving in either direction every T min. A girl is driving scooty with a speed of 60 km/h in the direction X to Y. She notices that a bus goes past her every 30 minutes in the direction of her motion, and every 10 minutes in the opposite direction. Choose the correct option for the period T of the bus service and the speed (assumed constant) of the buses.
- NEET (UG) - 2025
- Relative Velocity
- The intensity of transmitted light when a polaroid sheet, placed between two crossed polaroids at \(22.5^\circ\) from the polarization axis of one of the polaroids (\(I_0\) is the intensity of polarised light after passing through the first polaroid):
- NEET (UG) - 2025
- Polarization
A full wave rectifier circuit with diodes (\(D_1\)) and (\(D_2\)) is shown in the figure. If input supply voltage \(V_{in} = 220 \sin(100 \pi t)\) volt, then at \(t = 15\) msec:
- NEET (UG) - 2025
- Semiconductor Diode
Concepts Used:
Enzymes
Catalysts of biological systems can be known as Enzymes. Enzymes helps us to perform the huge variety of biochemical reactions that encompass in life are all mediated by a series of activities.
Properties of Enzymes:
Following are the properties of enzymes:
- Enzymes initiate and speed up the reaction
- The enzyme’s activity is ph dependent
- Enzymes have the capacity to forward and reverse the reaction but do not decide the direction of the biochemical pathway
- An enzyme has a particular area on which the substrate interacts to produce the desired product
- Enzyme becomes unstable at varying ph, high temperature and heat
- It have protein like characteristics
- Substrates can be catalyzed with a small number of enzymes
- Enzyme activity represents absolute, relative, group and stereo specificities
- Some enzymes perform regulatory function
- It minimise activation energy (Ea)
- The enzymes remain unaffected before and after the product formation and it can be reused
- They are soluble both in water and NaCl.